Capilliposide Isolated from Lysimachia capillipes Hemsl. Induces ROS Generation, Cell Cycle Arrest, and Apoptosis in Human Nonsmall Cell Lung Cancer Cell Lines
Figure 2
LC induces apoptosis in human NSCLC cells. (a) H460 cells treated with 2, 4 and 6 μg/mL LC for 24 hours and apoptosis rates were analyzed by flow cytometry after annexin V/PI staining. (b) Apoptosis was assessed by Hoechst 33342/PI double staining as described in Section 2. High blue fluorescent indicates apoptotic cells (pink arrow), low blue indicates live cells (azury arrow), while red represents dead cells (red arrow). Apoptosis was expressed as a percentage of the total number of nuclei examined. All data are representative of at least three independent experiments. and indicate statistically significant differences versus control group.
