Protective Effect of Ligustrazine on Lumbar Intervertebral Disc Degeneration of Rats Induced by Prolonged Upright Posture

Table 1

Sequences of primers used in the real-time RT-PCR.

Genes	Forward primer	Reverse primer
Col2α1 (112 bp)	F: 5′-TCCTAAGGGTGCCAATGGTGA-3′	R: 5′-GGACCAACTTTGCCTTGAGGAC-3′
MMP 13 (142 bp)	5′-CCCTGGAGCCCTGATGTTT-3′	5′-CTCTGGTGTTTTGGGGTGCT-3′
Col10α1 (143 bp)	5′-TTCACAAAGAGCGGACAGAGA-3′	5′-TCAAATGGGATGGGAGCA-3′
β-actin (150 bp)	5′-GGAGATTACTGCCCTGGCTCCTA-3′	5′-GACTCATCGTACTCCTGCTTGCTG-3′
iNOS (101 bp)	5′-CTCACTGTGGCTGTGGTCACCTA-3′	5′-GGGTCTTCGGGCTTCAGGTTA-3′
IL-1β (120 bp)	F: 5′-AGGTCGTCATC; ATCCCACGAG-3′	R: 5′-GCTGTGGCAG; CTACCTATGTCTTG-3′
COX-2 (145 bp)	F: 5′-GGAGCATCCTGAGTGGGATGA-3′	R: 5′-AAGCAGGTCTGGGTCGAACTTG-3′
MMP3 (104 bp)	F: 5′-TGGACCAGGG; ACCAATGGA-3′	R: 5′-GGCCAAGTTCA; TGAGCAGCA-3′

MMP-13: matrix metalloproteinase 13; IL-1β: interleukin 1β; COX-2: cyclooxygenase 2; MMP-3: matrix metalloproteinase 3; iNOS: inducible nitric oxide synthase 2.