Neuroprotective Effects of Jitai Tablet, a Traditional Chinese Medicine, on the MPTP-Induced Acute Model of Parkinson’s Disease: Involvement of the Dopamine System
Figure 4
JTT protected against MPTP-induced reductions in DAT levels of the mouse striatum as measured by immunohistochemistry and autoradiography. MPTP (20 mg/kg, 4 times, at 2-hour interval, i.p.) was injected on the 8th day of the 15-day experiment period. The animals were sacrificed on the 7th day after MPTP injections for immunohistochemistry and autoradiography studies. Photomicrographs of representative striatum immunohistochemistry sections (a) stained with a DAT antibody. Autoradiography of DAT binding using [125I]-β-CIT (c). A decrease in DAT levels was observed in MPTP mice. Treatment with JTT partially prevented this decrease. Optical density of DAT-positive fibers in the striatum (b), radioactivity binding of DAT in striatum (d) are expressed as the mean ± S.D, . *, ** versus vehicle group, # versus JTT-H.
