Herbal Prescription, DSGOST, Prevents Cold-Induced RhoA Activation and Endothelin-1 Production in Endothelial Cells
Figure 1
DSGOST inhibition of cold-induced responses in VSMC. (a) DSGOST inhibition of cold-induced RhoA activation in VSMC. Cells were pretreated with different concentrations (50, 100, 150, and 200 μg/mL) of DSGOST for 30 minutes and then exposed to cold temperature (25°C) for another 30 minutes. Active and total RhoA were blotted using anti-RhoA antibody. (b) DSGOST inhibition of endothelin-1-induced RhoA activation in VSMC. Cells were pretreated with 100 μg/mL of DSGOST for 30 minutes and then treated with 100 nmol/L of endothelin-1 for another 30 minutes. (c) Membrane translocation of α2c-adrenoreceptor. Cells were pretreated with DSGOST (100 μg/mL) for 30 minutes and then either exposed to cold (25°C) or treated with endothelin-1 (100 nmol/L) for 30 minutes. Plasma membrane protein was isolated and then α2c-adrenoreceptor was detected using the appropriate antibody. (d) RhoA-mediated α2c-adrenoreceptor translocation to the membrane. VSMC were transfected with CA-RhoA and then treated with DSGOST (100 μg/mL) for 30 minutes. α2c-Adrenoreceptor was detected in the plasma membrane protein pool. α1-Na+, K+-ATPase, was detected as the internal control for plasma protein.
