Herbal Prescription, DSGOST, Prevents Cold-Induced RhoA Activation and Endothelin-1 Production in Endothelial Cells
Figure 3
DSGOST inhibition of RhoA-mediated FAK phosphorylation. (a) HDMEC were pretreated with DSGOST (100 μg/mL) for 30 minutes and then exposed to cold (25°C) for another 30 minutes. F-actin was detected using rhodamine-phalloidin. The arrow indicates the representative of focal adhesion complex. Scale bars indicate 500 μm. (b) HDMEC were pretreated with DSGOST (100 μg/mL) for 30 minutes and then exposed to cold (25°C) for another 30 minutes. (c) HDMEC were transfected with CA-RhoA for 48 hours and then treated with DSGOST (100 μg/mL) for 30 minutes. (d) HDMEC were overexpressed with DN-RhoA and then exposed to cold (25°C) for another 30 minutes. Phosphorylated forms of FAK, SRC, and ERK were detected with appropriate antibodies. Actin was detected as the internal control.
