- About this Journal
- Abstracting and Indexing
- Aims and Scope
- Annual Issues
- Article Processing Charges
- Articles in Press
- Author Guidelines
- Bibliographic Information
- Citations to this Journal
- Contact Information
- Editorial Board
- Editorial Workflow
- Free eTOC Alerts
- Publication Ethics
- Reviewers Acknowledgment
- Submit a Manuscript
- Subscription Information
- Table of Contents
Evidence-Based Complementary and Alternative Medicine
Volume 2014 (2014), Article ID 605714, 6 pages
Chinese Medicine Shensongyangxin Is Effective for Patients with Bradycardia: Results of a Randomized, Double-Blind, Placebo-Controlled Multicenter Trial
1Department of Diagnosis, Shandong University Medical School, Jinan, Shandong 250014, China
2Research Center for Pathology and Physiology, Cardiovascular Institute and Fu Wai Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100037, China
3Integration of Traditional and Western Medical Research Academy of Hebei Province/Key Research Centre of Collateral Disease of State Administration of Traditional Chinese Medicine, Shijiazhuang, Hebei 050035, China
4Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250014, China
Received 28 September 2013; Accepted 26 November 2013; Published 16 January 2014
Academic Editor: Hongcai Shang
Copyright © 2014 Yunfang Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
To evaluate the efficacy and safety of Shensong Yangxin (SSYX) in patients with bradycardia arrhythmias, a randomized, double-blind, and placebo-controlled study was conducted. Patients with bradycardia were randomly assigned to receive either SSYX (trial group, ) or placebo (control group, ) for 4 weeks. ECG, 24-hour continuous ECG recording, echocardiography, and hepatic and renal function were evaluated at baseline and after treatment. Results showed that the average heart rate, the fastest heart rate, and the lowest heart rate in the trial group were all significantly higher than those in the control group at the end of treatment ( or 0.01, resp.). Compared with pretreatment, the average heart rate, the fastest heart rate, and the lowest heart rate in the trial group all increased significantly after treatment ( or 0.01, resp.). Both the efficacy and the symptom scores in the trial group were significantly better than those in the control group after treatment (both having ). No severe adverse effects were reported. In conclusion, SSYX treatment significantly increased the heart rate in patients with bradycardia without severe side effects. The exact mechanisms remain to be further explored.
Bradycardia is classified as a pulse rate below 60 beats per minute and is a common phenomenon in both healthy and disease conditions [1, 2]. Bradycardia may be a common manifestation of general conduction system disease or iatrogenic, due to medications used for atrial fibrillation rate control , which is characterized by a spectrum of arrhythmias including sinus bradycardia, sinus pauses, atrial fibrillation or flutter, and paroxysmal supraventricular tachycardia. The symptoms of bradyarrhythmia are most commonly intermittent syncope or presyncope but may be general and nonspecific. Some patients with sinus node disease are unable to appropriately increase their heart rate with exercise. Others may present with symptoms due to the underlying cause, such as myocardial infarction or drug toxicity.
Bradycardia can include sinus-node dysfunction (sick sinus syndrome) and atrioventricular block [4, 5]. The symptoms are often related to the bradycardia itself, as well as dysfunction of the autonomic nerve system (e.g., dizziness, fatigue, weakness, chest distress, or heart failure). In such cases, treatment targeted solely to correct bradycardia may not be effective in elimination of symptoms . Besides, currently available drugs (e.g., atropine, isoproterenol, and theophylline) used to increase sinus rhythm are not tolerated for long term because of their side effects [7–9]. Since overdrive suppression of sinus automaticity may result in long pauses, syncope often occurs when tachycardia terminates . Management for bradycardia-tachycardia syndrome is difficult because most antiarrhythmic drugs or the drugs that control the heart rate during tachycardia by blocking atrioventricular conduction such as β-adrenergic receptor blockers, calcium-channel blockers, or digitalis may lead to more severe bradycardia when the tachyarrhythmia terminates. For those patients, a cardiac pacemaker would usually be implanted before drug treatment . Consequently, effective drugs with low side effects are of high interest as alternatives for treating those patients.
Some traditional Chinese medicines have been used to treat the disease related to arrhythmia for thousands of years. Shensongyangxin (SSYX) is a traditional Chinese medicine developed originally for treating cardiac tachyarrhythmias and it is a compound of the traditional Chinese materia medica consisting of 12 ingredients including Panax ginseng, dwarf lilyturf tuber, and Nardostachys root. Previous data suggested that SSYX has actions that may be beneficial in the treatment of symptomatic bradycardia. Small clinical studies demonstrated that SSYX effectively reduced ventricular premature beat and prevented bradycardia [12, 13]. Whole cell patch clamping experiments revealed that SSYX was a multiple ion channel blocker . Preliminary studies suggested that SSYX can reduce the number of ventricular ectopic beats while mildly increasing the heart rate [15, 16]. However, extensive clinical trials have not been performed. Therefore, there are not evidence-based data from multicenter studies to confirm the clinical effects of SSYX. In order to further prove the effects of SSYX and cumulate the clinical data, a randomized, double-blind, placebo-controlled clinical study was designed to fully evaluate the efficacy and safety of SSYX in patients experiencing bradycardic arrhythmias.
2. Patients and Methods
2.1. Eligibility and Exclusion Criteria
This study was a randomized, double-blind, placebo-controlled, multicenter study. Patients with the age of 18 to 70 years accompanied by symptomatic bradycardia (average heart rate of 40~60 bpm) were enrolled to the study. However, patients indicated for permanent pacing therapy could be considered for the study only when the patients refused to do so.
Patients who met at least one of the following conditions were eligible: (1) sick-sinus syndrome with: average ventricular rate of 40 to 50 bpm; average ventricular rate of 50 to 60 bpm with the lowest rate less than 35 bpm accompanied by symptoms related to bradycardia; sinus rhythm or other escape rhythms in atrium, AV junction, or ventricle that occurred ≥2.0 s after the termination of tachyarrhythmia; (2) atrioventricular block (AVB): third degree AVB with documented asystole not longer than 2.5 seconds or escaped heart rate not slower than 40 bpm while awake; second degree AVB; first degree AVB with prolonged PR interval longer than 300 ms or with symptoms due to loss of atrioventricular synchrony; (3) chronic bifascicular and trifascicular block; (4) bradycardia-tachycardia syndrome.
Exclusion criteria included the following: (1) drug induced bradycardia; (2) endocrine or metabolism abnormalities and electrolyte disequilibrium; (3) acute myocardial infarction or unstable angina pectoris; (4) severe congestive heart failure (NYHA functional class III or IV); (5) uncontrolled hypertension; serious respiratory dysfunction or asthma; (6) liver, renal dysfunction; (7) primary hematopoietic system disease; (8) pregnant or child nursing; (9) allergy to study drugs; (10) patients enrolled to other drug studies; (11) blood pressure less than 90/60 mmHg; (12) bradycardia history shorter than 2 months; (13) syncope due to bradycardia; (14) R-R interval of ≥3.0 seconds; (15) patients who were currently treated with class I, III, or IV antiarrhythmic drugs were also excluded.
The study protocol was fully explained and written informed consent was obtained from each participant. The present study was approved by the Ethics Committee of Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, and the Ethics Committees of the other 10 hospitals.
2.2. Study Design
This randomized, double-blind, placebo-controlled, multicenter trial was conducted at 11 hospitals across the mainland of China, during October 2007 to July 2008. Patients were randomized using Q-DAS statistical software by random permuted blocks and stratified by centers; the locations were divided into 11 regions, to orally receive either SSYX or placebo 1.6 g tid for 4 weeks. The SSYX capsule and placebo were produced and tested by Yiling Pharmaceutical Corporation (Shijiazhuang, China). They were identical in size, weight, color, and taste. Compliance with the study medication was monitored by counting the capsules individually.
Group assignment for all subjects was determined using a random table prior to the initiation of the study. The sequence of assignments was unknown to any of the investigators. Each assignment was kept in a sealed envelope and the order in numeric number was shown on the outside of the envelope. Thus, the orders could not be changed. Envelopes were arranged in order. The principal investigators generated this random selection a few months before recruiting the first subject. All the evaluations were performed by physicians or nurses who were blinded to the treatment given, using the same set of questionnaires and guidelines.
For all patients, the medical history, physical examination, blood tests (including serum glucose, electrolyte, GPT, GOT, creatinine, and urea nitrogen), 12-lead ECG, 24-hour Holter recording, and echocardiogram were screened. Diagnosis of the bradycardia was confirmed by 24-hour Holter recording. Follow-up clinical visits were scheduled at 4 weeks. All measurements were performed independently by two researchers and the values averaged. For all assays the intraobserver and interobserver variation coefficients were less than 5%, respectively. The participants screening and enrollment flowchart was shown in Figure 1.
The minimum sample size required for the study was calculated by the following. Compared with placebo, the experimental medicine curative effect is about 60%, control medicine is about 40%, alpha is 0.05, and beta is 0.2 (study power 80%), estimate for 98 cases in each group. Considering the drop factors, the study was designed to enroll 280 cases (140 cases in the trial group and 140 cases in the control group).
2.3. Outcome Measures
The primary outcome was objective criterion obtained by 24-hour Holter recording and the secondary outcome was subjective criterion obtained by symptom scores.
The criteria for assessing the therapeutic effects of SSYX capsules were formulated according to the “Guiding Principles of Clinical Research on Treatment of Coronary Heart Disease with Traditional Chinese New Drugs” published in 2002  and the “Therapeutic Effects and ECG Evaluation Criteria for Treatment of Coronary Heart Disease” published in the Symposium for Coronary Heart Disease and Arrhythmia with integrated TCM and Western Medicine in 1998 .
The objective criteria for assessing the therapeutic effects of SSYX capsules on bradycardia were as follows according to the results obtained by 24-hour Holter recording. (1) A markedly effective response was defined as an increase of 20% or more in the average heart rate. (2) An effective response was defined as an increase from 10% to 20% in the average heart rate. (3) No effective response was defined as less than 10% increase or even decrease in the average heart rate.
The criteria for the symptom score depended on the frequency and degree of the symptoms which included palpitation, shortness of breath, fatigue, chest distress, agrypnia, and night sweat. (1) A markedly effective response was defined as the symptom scores decreased by >70%. (2) An effective response required a decrease from 30% to 70% in the symptom scores. (3) No effective response was defined as less than 30% decrease in the symptom scores.
2.4. Safety Monitoring
All patients underwent follow-up office visits weekly during therapy. At each follow-up, blood routine, transaminases, serum urine nitrogen (BUN), physical examination, echocardiography, and 12-lead ECG were measured and the incidence and severity of various side effects (i.e., nausea, vomiting, diarrhea, abdominal pain, headache, and dizziness) which may be associated with therapy were monitored.
2.5. Statistical Analysis
Continuous variables are given as mean ±SD. Comparison of the basic characteristics between the control group and the trial group was made using Student -test. As the data were normally distributed, the difference between the two groups in terms of the changes of Holter recording before and after treatment was analyzed by repeated measures ANOVA. Pearson Chi-Square test or Fisher’s exact test was used to compare categorical variables.
Calculations were performed with the statistical software SAS 9.1.3 (SAS Institute Inc., USA) and Q-DAS for Clinical Trial 3.0. (Q-DAS software technology, China). The SAS data sheets ITT (intention-to-treat was defined as patients who were treated at least once) and FAS (full analysis set was defined as the participants including eligible cases and drop-out cases) were used for efficacy and SS (safety set was defined as participants who received treatment and safety analysis at least once).
3.1. Patient Characteristics
From October 2007 to July 2008, 241 consecutive patients were enrolled in this prospective study from 11 hospitals across the mainland of China. Of the 241 patients enrolled, 124 patients were randomized to the trial group and 117 patients to the control group. Nine patients in the trial group and 13 patients in the control groups were lost to follow up. As a result, a total of 219 patients (115 in the trial group and 104 in the control group) completed the study. The main patient characteristics and the types of bradycardia were summarized in Table 1. The arms of the study were well balanced with respect to age, gender, height, weight, blood pressure, average heart rate (AHR), fastest heart rate (FHR), lowest heart rate (LHR), left ventricular ejection fraction, QT interval, NYHA class, ischemic heart disease, history of bradycardia, sinus bradycardia, sinus pauses, atrioventricular block, and bradycardia-tachycardia.
3.2. Changes of Heart Rate
One patient in SSYX group and 4 patients in placebo group refused to repeat 24-hour Holter recording at the end of treatment.
As shown in Table 2, the average heart rate in the trial group was significantly higher than that in the control group after treatment ( versus , ). As for the effect of SSYX on heart rate, we found that the 24-hour average heart rate increased by in the trial group and by in the control group after treatment (). Both the fastest heart rate and the lowest heart rate in the trial group were significantly higher than those in the control group at the end of 4 weeks ( or 0.01, resp.). Compared with pretreatment, the average heart rate, the fastest heart rate, and the lowest heart rate in the trial group all increased significantly after treatment ( or 0.01, resp.). The average heart rate and the lowest heart rate in the control group were also increased significantly at the end of 4 weeks ( or 0.01, resp.), whereas the fastest heart rate did not change significantly ().
3.3. Objective Efficacy
The results of objective efficacy were analyzed in all of the participants including eligible cases and drop-out cases. As shown in Table 3, the markedly effective response rate was 24.2% in the trial group and 0.9% in the control group after treatment. The effective response rates were 34.7% in the trial group and 18.8% in the control group, respectively. Significant differences were found between the two groups ().
3.4. Symptom Scores
The results of symptom scores were shown in Table 4. Compared with the baseline level, the symptom scores in both groups decreased after treatment (% versus in the trial group and % versus in the control group). Compared with the control group, the symptom score in the trial group was significantly lower ().
3.5. Adverse Events
Adverse events most possibly related to the treatment were reported in 1 (0.8%) patient in the trial group and 5 (4.3%) patients in the control group. Palpitation, headache, stomachache, and abdominal distension were the major symptoms. The symptoms completely recovered a few days after termination of the treatment. Neither death nor serious adverse event was reported during the study.
The current study was designed to evaluate the efficacy and safety of SSYX in the treatment of patients with bradycardic arrhythmias. The results demonstrated that SSYX treatment significantly increased heart rate in patients with bradycardia. The total effective rate was 63.5% in the trial group and 22.1% in the control group. Symptom scores were also significantly improved in the trial group compared with the control group. These results compare well with studies that suggested that SSYX has effect on paroxysmal atrial fibrillation  and premature ventricular contractions .
The effects of SSYX in treating cardiac rate and contraction abnormalities may be conferred by such ingredients of SSYX as Panax ginseng, which may balance the tension of autonomic nerve system . Our previous electrophysiological study demonstrated that SSYX increased heart rate and enhanced conducting capacity of the heart in the Chinese miniature swine only when the autonomic nervous system was intact . Another research has shown that SSYX affects cardiac action potentials by lowering the L type calcium channel currents and transient outward potassium currents in rabbit pulmonary vein myocytes , while a study on guinea pig ventricular myocytes showed that these channels were blocked  and another study found that SSYX could block these and many other ion channels such as the sodium current, the inward rectifier potassium current, and the delayed rectifier current, in rat and guinea pig ventricular myocytes .
It is earlier to come to a conclusion on the effect of SSYX on patients with II (type 2) or III degrees of atrioventricular block because of the small number of patients tested. For the sake of safety, patients with extreme bradycardia (e.g., heart rate below 40 beats per minute while awake, R-R interval exceeding 3.0 seconds in sinus rhythm, or experiencing syncope probably caused by bradycardia) were excluded in the trial. For those patients, implantation of a pacemaker is recommended if necessary.
There was no death or severe adverse events registered during the study. No significant changes in hepatic and renal function have been observed immediately after study. Neither proarrhythmic events nor QTc prolongation related to SSYX treatment were recorded. The most common adverse effects were temporary stomachache and abdominal distension. The outcomes of the study suggested that clinical use of SSYX was safe and effective for treating patients with sinus bradycardia.
There are some limitations in our study. First, the observation time is only 4 weeks and hence a longterm study is obviously needed to further confirm our results. Second, clinical and laboratory findings were used as the endpoints in our study, whereas a hard clinical endpoint such as mortality should be used in a large sample of patients in a future study. Third, a positive control is not set in this study and we will evaluate if SSYX is optimal efficiency or noninferiority in the future.
In conclusion, this study shows that SSYX is safe and effective for the treatment of bradycardia. This result means that SSYX should be considered an alternative treatment for bradycardic patients, especially those who are not recommended for pacemakers or patients who choose not to have a pacemaker fitted.
Conflict of Interests
The authors declare that there is no conflict of interests regarding the publication of this paper.
The study was supported by the National Basic Research Program (973 Program) of China (no. 2005CB523301, the Ministry of Science and Technology). The authors thank Mr./Ms. Xinchun Yang, director of Cardiac Center of Chaoyang Hospital of Capital Medical University, Xueqi Li, director of Cardiac Center of the 4th Hospital of Harbin Medical University, Xingui Guo, director of Cardiac Center of Shanghai Huandong Hospital, Lanjun Sun, director of Cardiac Center of the 2nd Hospital of Tianjin University of Chinese Medicine, Ming Chen, director of Cardiac Center of the 1st Hospital of Chongqing Medical University, Yabin Zhou, director of Cardiac Center of 1st Hospital of Heilongjiang University of Chinese Medicine, Xiling Shou, director of Cardiac Center of Shanxi province Hospital, Guang Fu, director of Cardiac Center of Changsha Hospital, Zhilin Miao, director of Shenyang Cardiac Hospital of Shenyang Medical College, Jingyuan Mao, director of Cardiac Center of 1st Hospital of Tianjin University of Chinese Medicine, and Xincan Liu, director of Cardiac Center of 1st Hospital of Henan University of Chinese Medicine, for his/her contribution as the person in charge of branch-center in the trial.
- R. G. Holly, J. D. Shaffrath, and E. A. Amsterdam, “Electrocardiographic alterations associated with the hearts of athletes,” Sports Medicine, vol. 25, no. 3, pp. 139–148, 1998.
- W. J. Groh, “Arrhythmias in the muscular dystrophies,” Heart Rhythm, vol. 9, no. 11, pp. 1890–1895, 2012.
- L. Feng, J. Gong, Z.-Y. Jin et al., “Electrophysiological effects of Chinese medicine Shen song Yang xin (SSYX) on Chinese miniature swine heart and isolated guinea pig ventricular myocytes,” Chinese Medical Journal, vol. 122, no. 13, pp. 1539–1543, 2009.
- D. Durham and L. I. Worthley, “Cardiac arrhythmias: diagnosis and management. The bradycardias,” Critical Care and Resuscitation, vol. 4, no. 1, pp. 54–60, 2002.
- J. W. Ufberg and J. S. Clark, “Bradydysrhythmias and atrioventricular conduction blocks,” Emergency Medicine Clinics of North America, vol. 24, no. 1, pp. 1–9, 2006.
- W. J. Brady Jr. and R. A. Harrigan, “Diagnosis and management of bradycardia and atrioventricular block associated with acute coronary ischemia,” Emergency Medicine Clinics of North America, vol. 19, no. 2, pp. 371–384, 2001.
- G. Das, “Cardiac effects of atropine in man: an update,” International Journal of Clinical Pharmacology Therapy and Toxicology, vol. 27, no. 10, pp. 473–477, 1989.
- L. Hendeles, M. Weinberger, G. Milavetz, M. Hill III, and L. Vaughan, “Food-induced “dose-dumping” from a once-a-day theophylline product as a cause of theophylline toxicity,” Chest, vol. 87, no. 6, pp. 758–765, 1985.
- D. P. Zipes, A. J. Camm, M. Borggrefe, et al., “ACC/AHA/ESC 2006 guidelines for management of patients with ventricular arrhythmias and the prevention of sudden cardiac death: a report of the American College of Cardiology/American Heart Association Task Force and the European Society of Cardiology Committee for Practice Guidelines,” Journal of the American College of Cardiology, vol. 48, no. 5, pp. e247–e346, 2006.
- W. S. Aronow, “Management of atrial fibrillation in the elderly,” Minerva Medica, vol. 100, no. 1, pp. 3–24, 2009.
- P. Rucinski, A. Rubaj, and A. Kutarski, “Pharmacotherapy changes following pacemaker implantation in patients with bradycardia-tachycardia syndrome,” Expert Opinion on Pharmacotherapy, vol. 7, no. 16, pp. 2203–2213, 2006.
- J. Z. Huang, H. Yan, X.G. Chen, and J. C. Liang, “Effect of Shensong Yangxin Capsule on ventricular premature beat and heart rate variablity,” Modern Medicine and Health, vol. 22, no. 18, pp. 2843–2843, 2006.
- X. X. Shen and W.X. Lu, “Effect of Shensong Yangxin Capsule on sinus node bradycardi,” Chinese Journal of Information on TCM, vol. 15, article 76, 2008.
- N. Li, K.-J. Ma, X.-F. Wu, Q. Sun, Y.-H. Zhang, and J.-L. Pu, “Effects of Chinese herbs on multiple ion channels in isolated ventricular myocytes,” Chinese Medical Journal, vol. 120, no. 12, pp. 1068–1074, 2007.
- S. Chai, S. Wang, L. Yao, A. Wu, Y. Liu, and C. Rao, “Effect of shensongyangxin capsule on myocardial remodeling and ventricular fibrillation threshold value in rat with coronary artery ligation,” Zhongguo Zhongyao Zazhi, vol. 34, no. 16, pp. 2101–2104, 2009.
- N. Li, Y.-P. Huo, K.-J. Ma, Q. Sun, and J.-J. Pu, “Effects of solution of dry powder of ShenSongYangXin capsule on sodium current and L-type calcium current in ventricular myocytes: experiment with guinea pig,” National Medical Journal of China, vol. 87, no. 14, pp. 995–998, 2007.
- K. J. Chen, J. Z. Liao, and Z. X. Xiao, Research on Cardiocerebrovascular Diseases, Shanghai Publishing Company of Science and Technology, Shanghai, China, 1998.
- A.-H. Wang, J.-L. Pu, X.-Y. Qi et al., “Evaluation of shensongyangxin capsules in the treatment of paroxysmal atrial fibrillation: a randomized, double-blind and controlled multicenter trial,” Zhonghua yi Xue Za Zhi, vol. 91, no. 24, pp. 1677–1681, 2011.
- J.-G. Zou, J. Zhang, Z.-H. Jia, and K.-J. Cao, “Evaluation of the traditional chinese medicine shensongyangxin capsule on treating premature ventricular contractions: a randomized, double-blind, controlled multicenter trial,” Chinese Medical Journal, vol. 124, no. 1, pp. 76–83, 2011.
- A. Zheng and T. Moritani, “Effect of the combination of ginseng, oriental bezoar and glycyrrhiza on autonomic nervous activity and immune system under mental arithmetic stress,” Journal of Nutritional Science and Vitaminology, vol. 54, no. 3, pp. 244–249, 2008.
- V. Essebag, T. Hadjis, R. W. Platt, and L. Pilote, “Amiodarone and the risk of bradyarrhythmia requiring permanent pacemaker in elderly patients with atrial fibrillation and prior myocardial infarction,” Journal of the American College of Cardiology, vol. 41, no. 2, pp. 249–254, 2003.
- L. Shi, X.-C. Yang, X.-L. Liu, M. Zong, and Y.-L. Wu, “Effects of ShenSongYangXin on action potential and some current channels in isolated rabbit pulmonary vein cardiomyocytes,” Zhonghua yi Xue Za Zhi, vol. 89, no. 30, pp. 2142–2146, 2009.