Ligusticum wallichii Extract Inhibited the Expression of IL-1β after AMI in Rats
Figure 3
Ligusticum wallichii extract inhibited the expression of IL-1β. (a) Representative micrographs were taken at a magnification of ×400. The brown areas were IL-1β positive cell; the brown areas were significantly increased in the AMI model group. Ligusticum wallichii and aspirin decreased the brown area. (b) In the sham group, the immunoreactive staining occurred less in the cytoplasm. The number of IL-1β-like immunoreactive cells increased significantly in the myocardium after AMI when compared with the sham group . Ligusticum wallichii (10 mg/Kg/d body weight) and aspirin (10 mg/Kg/d body weight) both inhibited IL-1β protein expression versus AMI group , but there were no remarkable differences between LW group and aspirin group. (c) Serum IL-1β level was assessed by ELISA kit. The serum IL-1β level was significantly upregulated 1w after AMI when compared with the sham group . Ligusticum wallichii (10 mg/Kg/d body weight) and aspirin (10 mg/Kg/d body weight) both inhibited the IL-1β overexpression after AMI versus AMI model , but there were no remarkable differences between LW group and aspirin group. (d) Effect of Ligusticum wallichii on the IL-1β mRNA expression in the hypothalamus of AMI rats. Quantitative RT-PCR was performed. The IL-1β mRNA expression was significantly upregulated 1w after AMI versus sham group . Ligusticum wallichii and aspirin inhibited the hypothalamus IL-1β mRNA expression versus AMI group , but there were no remarkable differences between LW group and aspirin group.
