Novel Antidepressant-Like Activity of Caffeic Acid Phenethyl Ester Is Mediated by Enhanced Glucocorticoid Receptor Function in the Hippocampus

Figure 3

Differential GR phosphorylation by CAPE treatment. Following the FST, the hippocampus was dissected and frozen in dry ice. (a) Representative Western blot images (upper panel) and (b) quantitation (lower panel) depicting pGR(S220), pGR(S234), and GR expression in hippocampal lysates. The average band intensity of the pGRs was normalized by total GR protein. (c) The pGR S220/S234 ratio. The columns and error bars represent means ± SEM ( per group). Data were analyzed using a one-way ANOVA followed by Tukey’s post hoc test. versus VEH. (d) Pearson correlation analysis between the pGR(S220)/(S234) ratio and time spent immobile in the TST. Scatter plots with fitted linear regression lines depict significant correlations. Each of the eight points corresponds to VEH (open circle) and CAPE 20 μmol/kg (closed circle), respectively. The squared correlation coefficient () is provided above the plot.

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