Pien Tze Huang Overcomes Multidrug Resistance and Epithelial-Mesenchymal Transition in Human Colorectal Carcinoma Cells via Suppression of TGF-β Pathway

Figure 6

Effect of PZH on the expression of ABCG2, EMT-related factors, and activation of TGF-β pathway in HCT-8/5-FU cells. HCT-8/5-FU cells were treated with indicated concentrations of PZH for 24 h. The mRNA (a) and protein (b) expression levels of ABCG2, E-cadherin, N-cadherin, ZEB1, ZEB2, TGF-β, and SMAD4 in HCT-8/5-FU cells were determined by RT-PCR and Western Blot analyses. GAPDH or β-actin was used as the internal control for RT-PCR or Western Blot, respectively. Images are representatives of three independent experiments.

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