Validation of the Antidiabetic and Hypolipidemic
Effects of Clitocybe nuda by Assessment of Glucose
Transporter 4 and Gluconeogenesis and AMPK
Phosphorylation in Streptozotocin-Induced Mice
Figure 4
Semiquantitative RT-PCR analysis on (a) PEPCK, (b) 11-HSD1, (c) G6Pase, (d) PPAR, (e) ATGL, and (f) DGAT2 mRNA expression in liver tissue of the mice by oral gavage extracts of Clitocybe nuda (C1: 0.2, C2: 0.5, and C3: 1.0 g/kg bodyweight); Metf: metformin (0.3 g/kg body weight). Total RNA (1 g) isolated from tissue was reverse transcripted by MMLV-RT; 10 L RT products were used as templates for PCR. The expression levels of PEPCK, 11-HSD1, G6Pase, PPAR, ATGL, and DGAT2 mRNA were measured and quantified by image analysis. Values were normalized to GAPDH mRNA expression. All values are means S.E. (). #P < 0.05, ##P < 0.01, and ###P < 0.001 compared with the control (CON) group; *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the streptozotocin + vehicle (distilled water) (STZ) group.
