Validation of the Antidiabetic and Hypolipidemic Effects of Clitocybe nuda by Assessment of Glucose Transporter 4 and Gluconeogenesis and AMPK Phosphorylation in Streptozotocin-Induced Mice

Figure 5

The phospho-AMPK (Thr172) protein contents in (a) liver and (b) skeletal muscle and (c) GLUT4 protein contents inskeletal muscle of the mice by oral gavage extract of Clitocybe nuda. Protein was separated by 12% SDS-PAGE detected by western blot. All values are means ± S.E. (𝑛=9). ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 compared with the control (CON) group; *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the streptozotocin + vehicle (distilled water) (STZ) group. C1, C2, and C3: extracts of Clitocybe nuda (C1: 0.2, C2: 0.5, and C3: 1.0 g/kg bodyweight); Metf: metformin (0.3 g/kg body weight).

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