Validation of the Antidiabetic and Hypolipidemic Effects of Clitocybe nuda by Assessment of Glucose Transporter 4 and Gluconeogenesis and AMPK Phosphorylation in Streptozotocin-Induced Mice

Table 2

Absolute tissue weight, weight gain over 4-week treatment (g), and blood profiles.

Parameter	CON	STZ	STZ + C1	STZ + C2	STZ + C3	STZ + Metf
			0.2^a	0.5^a	1.0^a	0.3^a
Absolute tissue weight (g)
EWAT	0.235±0.009	0.075±0.007^###	0.058±0.010	0.061±0.005	0.067±0.011	0.090±0.010
MWAT	0.305±0.014	0.216±0.011^###	0.197±0.018	0.237±0.017	0.247±0.015	0.250±0.014
RWAT	0.024±0.002	0.016±0.009	0.007±0.002	0.007±0.003	0.005±0.001	0.012±0.003
Visceral fat	0.259±0.010	0.091±0.012^###	0.065±0.011	0.070±0.007	0.072±0.012	0.102±0.012
BAT	0.081±0.010	0.100±0.049	0.056±0.004	0.044±0.006	0.052±0.003	0.048±0.003
Liver (g)	0.992±0.032	1.031±0.048	1.048±0.031	1.063±0.064	1.121±0.049	1.074±0.020
Spleen	0.073±0.004	0.055±0.003^#	0.069±0.009	0.061±0.006	0.056±0.002	0.055±0.003
Skeletal muscle	0.270±0.021	0.175±0.012^###	0.155±0.014	0.147±0.016	0.175±0.012	0.183±0.005
Weight gain (g)	0.10±0.32	−0.19±0.73	−0.92±0.20	−1.24±0.35	−0.58±0.70	−0.79±0.67
Blood profiles
TC (mg/dL)	95.0±5.9	140.7±7.3^###	160.0±10.4	157.5±6.2	153.3±6.8	114.3±7.8^∗
Insulin (𝜇U/mL)	10.27±0.59	4.07±0.38^###	8.88±0.81^∗∗	11.08±1.87^∗∗	13.29±0.79^∗∗∗	11.25±1.67^∗∗

All values are means ± S.E. (𝑛=9). ^#𝑃<0.05, ^##𝑃<0.01, and ^###𝑃<0.001 compared with the control (CON) group; ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001 compared with the streptozotocin (STZ) + vehicle (distilled water) (STZ) group. C1, C2, and C3: extracts of Clitocybe nuda. BAT: brown adipose tissue; EWAT: epididymal white adipose tissue; RWAT: retroperitoneal white adipose tissue; MWAT: mesenteric white adipose tissue; EWAT + RWAT: visceral fat; TC: total cholesterol; TG: triglyceride.
^aDose (g/kg/day).