Caffeamide 36-13 Regulates the Antidiabetic and Hypolipidemic Signs of High-Fat-Fed Mice on Glucose Transporter 4, AMPK Phosphorylation, and Regulated Hepatic Glucose Production

Figure 5

The protein contents of GLUT4 in skeletal muscle, the ratio of phospho-AMPK (Thr172) to total AMPK in liver tissue and skeletal muscle, and quantified results for the phosphorylation status of Akt (p-Akt normalized to total Akt (pAkt/Akt)) in liver tissue of the mice by oral gavage caffeamide 36-13 (TS) for 4 weeks. Protein was separated by 12% SDS-PAGE detected by western blot. All values are means ± S.E. (𝑛=9). ^#𝑃<0.05, ^##𝑃<0.01, ^###𝑃<0.001 compared with the control (CON) group; ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001 compared with the high-fat + vehicle (HF) group. TS: T1: 10, T2: 20 mg/kg bodyweight; Rosi: rosiglitazone (0.01 g/kg body weight).

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