Anti-Inflammatory and Antihyperalgesic Activities of Ethanolic Extract and Fruticulin A from Salvia lachnostachys Leaves in Mice

Figure 5

Effect of oral administration of fruticulin A at inhibition of the leukocyte migration (a) and protein extravasation (b) on pleurisy test in mice. Mice were treated one hour before an intrapleural injection of carrageenan, with fruticulin A (0.3, 1, and 3 mg/kg p.o.), dexamethasone (DEXA, 1 mg/kg, s.c.), or saline solution (V). Naive group (N), also treated with saline p.o., received an intrapleural injection of sterile saline. Pleural cavity was washed with PBS/EDTA 10 mM. Cells were counted and plasma leakage was analyzed. In (a), number of cells that migrated to pleural cavity 4 hours after carrageenan injection. In (b), plasma leakage measured by Bradford’s reaction. The symbol compared vehicle (V) versus fruticulin A or DEXA treated groups and compared the vehicle and treated group with naive. ; ; . Differences between groups were analyzed by analysis of variance (one-way ANOVA) followed by the Newman-Keuls test.

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