Beneficial Effects of Fractions of Nardostachys jatamansi on Lipopolysaccharide-Induced Inflammatory Response
Figure 6
Effects of NJ fractions on NF-κB activation. ((a)–(d)) Peritoneal macrophages were treated with NJ fractions (100 μg/mL) for 1 h and then stimulated with LPS (500 ng/mL) at indicated time points. The cells were harvested for western blot. Total cellular proteins (20 μg) were resolved by SDS-PAGE, transferred to PVDF membrane, and detected with Iκ-Bα (40 kDa) antibody. -Actin was used as loading control. A representative western blot of three experiments is shown. (e) Peritoneal macrophages were treated with NJ fractions (100 μg/mL) for 1 h and then stimulated with LPS (500 ng/mL) for 1 h. Then, nuclear translocation of NF-κB p65 was examined using immunostaining. NF-κB p65 was shown as red, and the nucleus was shown as blue (DAPI). The values are means ± S.E. of three independent experiments. versus saline. versus LPS.
