Integrated Analysis for Identifying Radix Astragali and Its Adulterants Based on DNA Barcoding

Table 6

Six indicators assessed for DNA barcoding.

DNA barcodes	Parameters
	Average genetic distance	Identification efficiency		Gap rate	Inter- to intraspecific variation	Barcoding gap	Total score
		BLAST1	Nearest distances
ITS2	8	12	8	8	8	4	48
ITS	6	28	22	0	0	6	62
psbA-trnH	6	26	18	0	2	2	54
rbcL	4	12	14	4	6	8	48
matK	4	14	24	4	4	2	52
COI	2	6	10	6	0	6	30

*The total score of six parameters was set by 10, 30, 30, 10, 10, and 10 in order. Identification efficiency based on two methods was set by 30 score because of its importance for identification.