Rabdosia japonica var. glaucocalyx Flavonoids Fraction Attenuates Lipopolysaccharide-Induced Acute Lung Injury in Mice

Figure 3

Effects of RJFs on the lung W/D ratio, NO, total protein concentration in BALF, and the level of C3 in serum of mice. 2 h after RJFs pretreatment on day 7, mice were slightly anesthetized, and then 2 mg/kg LPS was instilled intratracheally (i.t.) to induce lung injury. The lung W/D ratio was determined at 6 h after LPS challenge; BALF and the serum were collected at 6 h following LPS challenge. NO and total protein concentration in the supernatant of BALF were determined by kits. C3 activity in serum was determined by mouse C3 ELISA kit. Data expressed as means ± S.D. (𝑛=10); ^##𝑃<0.01 compared with control, ^∗𝑃<0.05 and ^∗∗𝑃<0.01 compared with vehicle treated ALI model group.

(a)

(b)

(c)

(d)