Research Article
The Effect of Temperature on Pressurised Hot Water Extraction of Pharmacologically Important Metabolites as Analysed by UPLC-qTOF-MS and PCA
Table 1
List of putatively annotated pharmacologically relevant metabolites extracted from M. oleifera using the PHWE technique.
| | Compound name | Rt (min) | [M-H]− | Molecular formula |
| | 3-Caffeoylquinic acid (3-CQA) | 4.31 | 353.0853 | | | 4-Caffeoylquinic acid (4-CQA) | 5.93 | 353.0859 | | | 3-cis-p-Coumaroylquinic acid (3-pCoQA) | 5.39 | 337.0896 | | | 3-trans-p-Coumaroylquinic acid (3-pCoQA) | 5.50 | 337.0899 | | | 4-cis-p-Coumaroylquinic acid (4-pCoQA) | 7.67 | 337.0905 | | | 4-trans-p-Coumaroylquinic acid (4-pCoQA) | 7.75 | 337.0931 | | | 3-cis-Feruloylquinic acid (3-FQA) | 6.13 | 367.1012 | C17H20O9 | | 3-trans-Feruloylquinic acid (3-FQA) | 6.38 | 367.10428 | C17H20O9 | | 4-Feruloylquinic acid (4-FQA) | 9.30 | 367.1048 | C17H20O9 | | Caffeic acid | 5.88 | 179.0331 | | | Quinic acid (QA) | 0.96 | 191.0165 | C7H12O6 | | Apigenin-7-glycoside | 8.46 | 593.1523 | C27H30O15 | | Quercetin 3-glycosides; monosaccharides, 3-O-(6-O-malonyl-β-D-galactopyranoside) | 17.49 | 549.2561 | | | Quercetin 3-glycosides; monosaccharides, 3-O-β-D-glucopyranoside | 13.91 | 463.0798 | | | Kaempferol 3-glycosides; monoglycosides, 3-O-(6-O-malonyl-β-D-galactopyranoside) | 18.64 | 533.0915 | | | Kaempferol 3-glycosides; monoglycosides, 3-O-a-D-galactopyranoside | 17.02 | 447.0840 | | | Quercetin 3-glycosides; monosaccharides, 3-O-[4-carboxy-3-hydroxy-3-methylbutanoyl-(→6)-β-D-galactopyranoside] | 16.27 | 607.1305 | | | Kaempferol | 21.06 | 285.0410 | | Isorhamnetin 3-glycosides; monosaccharides, 3-O-(6-O-acetyl-β-D-glucopyranoside)
| 18.29 | 519.1061 | | | Quercetin 3-glycosides; monosaccharides, 3-O-(6-O-acetyl-a-D- glucopyranose) | 17.18 | 533.1090 | |
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Extracted more with 50°C.
**Extracted more with 150°C.
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