Evidence-Based Complementary and Alternative Medicine

Research Article

Gene Expression Profiles Underlying Selective T-Cell-Mediated Immunity Activity of a Chinese Medicine Granule on Mice Infected with Influenza Virus H1N1

Table 3

Genes associated with antigen processing presentation.


Symbol	GenBank accession	Gene description	log₂ FC^a
Symbol	GenBank accession	Gene description	M/N	SH/M	SM/M	SL/M	Oseltamivir/M

Tap1	PH_mM_0000666	Transporter 1, ATP-binding cassette, subfamily B (MDR/TAP)	2.24	−0.30	−2.83	−0.41	−1.00
Tap2	mMC017161	Transporter 2, ATP-binding cassette, subfamily B (MDR/TAP)	2.29	−0.02	−2.27	−0.20	−1.05
Cd74	PH_mM_0016294	CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), also as CLIP	1.74	0.47	0.60	0.13	−0.75
Ctsb	PH_mM_0013880	Cathepsin B	1.47	−0.27	−0.95	−0.24	−0.54
Ctss	PH_mM_0009536	Cathepsin S	1.99	0.29	−1.39	−0.28	−0.90
Hspa5	mMC021274	Heat shock protein 5	1.27	0.78	0.22	0.29	−0.16
Hspa1a	PH_mM_0000552	Heat shock protein 1A	2.05	−0.17	−2.39	−0.67	−0.75
Hspa1b	PH_mM_0001288	Heat shock protein 1B	1.53	0.63	−1.08	−0.47	−0.18
H2-M2	PH_mM_0002687	Histocompatibility 2, M region locus 2	2.27	−0.64	−2.16	−1.89	−1.47
H2-M3	PH_mM_0009138	Histocompatibility 2, M region locus 3	2.37	−0.24	−2.10	−0.55	−1.39
H2-Oa	PH_mM_0000005	Histocompatibility 2, O region alpha locus	1.15	−0.38	−0.44	−0.55	−0.67
H2-Q10	PH_mM_0005188	Histocompatibility 2, Q region locus 10	5.62	−2.04	−2.72	−3.84	−4.12
H2-Dma	PH_mM_0005361	Histocompatibility 2, class II, locus DMa	1.49	−0.17	−1.11	−0.43	−1.14
H2-Eb1	PH_mM_0006643	Histocompatibility 2, class II antigen E beta	1.30	−0.31	−1.87	−1.31	−1.33
H2-Ab1	PH_mM_0006906	Histocompatibility 2, class II antigen A, beta 1	1.17	0.26	−0.05	0.33	−0.10
Lgmn	mMC007543	Legumain	2.27	0.08	−1.10	−0.24	−0.83
Lta	mMC008431	Lymphotoxin A, TNF-β	1.34	−0.14	−1.17	−0.18	−1.19
Psme1	PH_mM_0004486	Proteasome (prosome, macropain) 28 subunit, alpha	1.00	−0.40	−1.16	−0.53	−0.50
H2-T24	PH_mM_0007562	Histocompatibility 2, T region locus 24	2.82	−0.15	−2.19	0.21	−1.72
Psme2	PH_mM_0012374	Proteasome (prosome, macropain) 28 subunit, beta	1.99	−1.00	−1.55	−1.58	−1.00
Cd8a	PH_mM_0015300	CD8 antigen, alpha chain	2.27	1.20	−1.65	−0.42	−1.05
Cd8b1	mMC017400	CD8 antigen, beta chain 1	2.49	1.31	−1.18	−1.58	−0.73
Cd4	mMC002333	CD4 antigen	1.05	−0.32	−1.11	−0.92	−1.00

Fold change. The intensity ratio of probe signal criteria for differential expressions was defined as a twofold change (up or down) (log₂ FC ≥ 1 or ≤−1) in gene expression compared with virus control group and a value less than 0.05 in at least one time point as a minimum requirement to select. For instance, log₂ (M/N) ≥ 1 means that the comparison between normal control group and virus control group was scattered, indicating that a large number of genes in virus control group were up-regulated in response to H1N1 infection. log₂ (SH/M) ≤−1, log₂ (SM/M) ≤ −1, log₂ (SL/M) ≤ −1, and log₂ (Oseltamivir/M) ≤ −1 means that down-regulation in gene expression attributable to the high-dose SFXF group treatment, medium-dose group SFXF treatment, low-dose SFXF group treatment, and Oseltamivir group treatment was done by comparing genes in virus control group, respectively ( value data not shown).