Antidiabetic and Antihyperlipidemic Effects of Clitocybe nuda on Glucose Transporter 4 and AMP-Activated Protein Kinase Phosphorylation in High-Fat-Fed Mice
Figure 3
Effects of the extract of Clitocybe nuda on liver tissue morphology in the low-fat (Con), high-fat (HF), HF + C1, HF + C2, HF + C3, or HF + Rosi groups. Pictures of hematoxylin and eosin-stained sections of (a) epididymal adipocytes (magnification: 10 (ocular) × 20 (object lens)) from mice fed with extract of Clitocybe nuda. White adipose tissue (named adipocytes) is polyhedral by H&E stain, and the appearance showed string-like cytosol surrounding a vacuole. This is because they were embedded in paraffin as immersed in lipid solvents, and finally all the fats were removed. Unobvious nucleus (N) in the other side of cells was carefully observed, and (b) liver tissue (magnification: 10 (ocular) × 40 (object lens)) was obtained from mice fed with extract of Clitocybe nuda. The high-fat diet induced the hepatic ballooning degeneration in the HF group as compared with the CON group. The ballooning degeneration is a form of liver parenchymal cell death and the nucleolus was squeezed into the other side named balloon (as the arrow indicated). This may be due to the heap of glycogen in the nucleus. High-fat diet induced obesity and insulin resistance. Insulin levels affected the storage of hepatic glycogen. Treatment with C2 and C3 significantly decreased the degree of ballooning degeneration. Each presented is typical and representative of nine mice. C1, C2, and C3: extracts of Clitocybe nuda (C1: 0.2, C2: 0.5, and C3: 1.0 g/kg body weight); Rosi: rosiglitazone (0.01 g/kg body weight).
