Antidiabetic and Antihyperlipidemic Effects of Clitocybe nuda on Glucose Transporter 4 and AMP-Activated Protein Kinase Phosphorylation in High-Fat-Fed Mice
Figure 4
Semiquantative RT-PCR analysis on (a) ATGL, (b) G6Pase, (c) PPARα, and (d) SREBP1c mRNA expressions in liver tissue of the mice by oral gavage extracts of Clitocybe nuda (C1: 0.2, C2: 0.5, and C3: 1.0 g/kg body weight); Rosi: rosiglitazone (0.01 g/kg body weight). Total RNA (1 μg) isolated from tissue was reverse transcripted by MMLV-RT, and 10 μL of RT products was used as template for PCR. The expression levels of ATGL, G6Pase, PPARα, and SREBP1c mRNA were measured and quantified by image analysis. Values were normalized to GAPDH mRNA expression. All values are mean S.E. (). and were compared with those of the control (CON) group; and were compared with those of the high-fat + vehicle (distilled water) (HF) group.
