The Resin from Protium heptaphyllum Prevents High-Fat Diet-Induced Obesity in Mice: Scientific Evidence and Potential Mechanisms

Figure 7

Effect of RPH on the expression of proteins PPARγ, C/EBPβ, and C/EBPα and in 3T3-L1 cells. β-actin protein was used as control. Postconfluent 3T3-L1 cells were differentiated in the absence or presence of RPH 12.5, 25, and 50 μg/mL for 11 days. Proteins expression was evaluated by Western blot analysis (a). Bar graphs represent densitometric results of bands PPARγ (b), C/EBPβ (c), and C/EBPα (d). UC indicates undifferentiated cell control and DC indicates differentiated cell control. While RPH significantly attenuated the expression levels of C/EBPβ at all concentration levels, statistically significant suppression was noticed for PPARγ at 25 and 50 μg/mL and for C/EBPα at 50 μg/mL only. Values are shown as mean ± SEM of three independent experiments. compared to untreated cell control (day 0).

(a)

(b)

(c)

(d)