Bathing Effects of Various Seawaters on Allergic (Atopic) Dermatitis-Like Skin Lesions Induced by 2,4-Dinitrochlorobenzene in Hairless Mice

Figure 1

Experimental designs used in this study. AD-like dermatitis was induced by sensitization of 1% DNCB once a day for 1 week and boosted by 0.5% DNCB, three times a week for 28 days. Each of eight mice per group was freely bathing 20 min/day. 1% DEXA was topically applied on the dorsal back skins once a day for 6 weeks from 5 weeks after DNCB sensitization. Intact and DNCB control mice were bathing on the distilled water, instead of seawaters to provide the same swimming stresses. Six weeks of bathing periods in this study were selected according to previous bathing effects of mineral-rich water.