Bathing Effects of Various Seawaters on Allergic (Atopic) Dermatitis-Like Skin Lesions Induced by 2,4-Dinitrochlorobenzene in Hairless Mice

Figure 2

Body weights changes during 6 weeks of continuous bathing on seawaters or topical application of DEXA in DNCB-induced AD mice. Significant () decreases of body weights were demonstrated in DNCB control mice at 6 and 7 days after initial DNCB sensitization as compared with intact vehicle control mice (a, b), but significantly () increased body weights were transiently noticed at 3 weeks after first DNCB boosting in DNCB control mice as compared with intact vehicle control mice (c). In addition, topical application of 1% DEXA and bathing on the all four different seawaters also did not influence the body weights as compared with those of DNCB control mice, throughout all experimental periods. Values are expressed as mean ± SD of eight hairless mice. AD = allergic/atopic-like dermatitis; DNCB = 2,4-dinitrochlorobenzene; DEXA = dexamethasone-water soluble; WSSW = west surface seawater collected around Wepo-ri (Ganghwa-do, Republic of Korea); WSGW = west saline groundwater collected at Yonggungoncheon (Seokmo-do, Republic of Korea); ESSW = east surface seawater collected around Nagok-ri (Uljin, Republic of Korea); ESGW = east saline groundwater collected around Hoojeong-ri (Uljin, Republic of Korea). Before mean 1 day before initial DNCB sensitization application; the day of 7 means start day of DNCB sensitization; the day of 35 means start day of bathing or topical treatment of DEXA. All animals were overnight fasted before sacrifice (arrow).