Bathing Effects of Various Seawaters on Allergic (Atopic) Dermatitis-Like Skin Lesions Induced by 2,4-Dinitrochlorobenzene in Hairless Mice

Figure 7

Representative histological images of splenic tissues, taken from unexposed intact or DNCB-induced AD mice bathing on seawaters or topical application of DEXA. Marked hypertrophic changes due to hyperplasia of red pulp lymphoid cells were detected on the splenic tissues in DNCB control mice as compared with intact vehicle control hairless mice, respectively. However, these hypersensitivities related splenic hypertrophic signs were significantly inhibited by topical treatment of 1% DEXA and bathing on the ESGW, WSGW, ESSW, and WSSW as compared with DNCB control mice, in that order, respectively. No meaningful changes on the numbers of white pulps were demonstrated by treatment of DNCB as compared with intact vehicle control and also by topical application of 1% DEXA or bathing on the all four types of seawaters as compared with DNCB control mice, in this experiment. (a) Intact vehicle control mice bathing on the distilled water; (b) DNCB control mice bathing on the distilled water; (c) AD mice bathing on the WSSW; (d) AD mice bathing on the WSGW; (e) AD mice bathing on the ESSW; (f) AD mice bathing on the ESGW; (g) 1% DEXA topically applied AD mice. AD = allergic/atopic-like dermatitis; DNCB = 2,4-dinitrochlorobenzene; DEXA = dexamethasone-water soluble; WSSW = west surface seawater collected around Wepo-ri (Ganghwa-do, Republic of Korea); WSGW = west saline groundwater collected at Yonggungoncheon (Seokmo-do, Republic of Korea); ESSW = east surface seawater collected around Nagok-ri (Uljin, Republic of Korea); ESGW = east saline groundwater collected around Hoojeong-ri (Uljin, Republic of Korea); WP = white pulp; RP = red pulp. All being hematoxylin-eosin stain. Scale bars = 400 μm.

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