Bathing Effects of Various Seawaters on Allergic (Atopic) Dermatitis-Like Skin Lesions Induced by 2,4-Dinitrochlorobenzene in Hairless Mice

Table 6

Changes on the skin mRNA expressions after 6 weeks of continuous bathing on seawaters or topical application of DEXA in DNCB-induced AD mice.

Groups	Skin mRNA expressions (relative expressions/GAPDH mRNA)
	Spleen	Submandibular LN	Spleen	Submandibular LN
Controls
Intact	1.03 ± 0.08	1.01 ± 0.05	1.04 ± 0.11	1.01 ± 0.08
DNCB	5.62 ± 1.22^a	4.94 ± 0.74^a	5.50 ± 1.14^a	3.07 ± 0.54^a
Reference
DEXA	2.74 ± 1.20^ab	1.88 ± 0.56^ab	2.25 ± 0.52^ab	1.40 ± 0.21^ab
Bathing on
WSSW	4.18 ± 0.52^ac	3.87 ± 0.60^ac	3.95 ± 0.89^ab	2.30 ± 0.37^ab
WSGW	3.46 ± 0.77^ac	3.06 ± 0.50^ab	3.47 ± 0.82^ab	1.98 ± 0.26^ab
ESSW	3.94 ± 0.62^ac	3.59 ± 0.66^ab	3.70 ± 0.71^ab	2.16 ± 0.28^ab
ESGW	2.82 ± 1.22^ac	2.55 ± 0.79^ab	3.13 ± 0.63^ab	1.74 ± 0.34^ab

Values are expressed as mean ± SD of eight hairless mice. AD = allergic/atopic-like dermatitis; DNCB = 2,4-dinitrochlorobenzene; DEXA = dexamethasone-water soluble; WSSW = west surface seawater collected around Wepo-ri (Ganghwa-do, Republic of Korea); WSGW = west saline groundwater collected at Yonggungoncheon (Seokmo-do, Republic of Korea); ESSW = east surface seawater collected around Nagok-ri (Uljin, Republic of Korea); ESGW = east saline groundwater collected around Hoojeong-ri (Uljin, Republic of Korea); GAPDH = glyceraldehyde 3-phosphate dehydrogenase. ^a as compared with intact control by MW test; ^b and ^c as compared with DNCB control by MW test.