Flavonoid 4′-O-Methylkuwanon E from Morus alba Induces the Differentiation of THP-1 Human Leukemia Cells

Figure 6

Increased expression of differentiation-associated surface antigen CD11b in 4ME-treated cells. THP-1 cells were cultured with indicated concentrations of 4ME or 1 µM ATRA, added as a positive control for 72 and 96 h. The level of expression of the indicated phenotypic marker was determined by flow cytometry using fluorescein-labelled monoclonal antibody against CD11b. The amount of cell-associated fluorescence in median fluorescence intensity (MFI) is illustrated in the graph. The results shown are expressed as the mean ± SD of three independent experiments, with each condition tested in duplicate.