Ginkgolide C Suppresses Adipogenesis in 3T3-L1 Adipocytes via the AMPK Signaling Pathway

Figure 5

Effects of ginkgolide C on the AMPK pathway in differentiated 3T3-L1 adipocytes. (a) Differentiated 3T3-L1 cells were treated with various concentrations of ginkgolide C for 24 h, and Sirt1, phosphorylation of AMPKα, AMPKα, phosphorylation of ACC-1, and ACC-1 were detected by western blot ( per group). AMPKα or ACC-1 expression was used as an internal control. (b) Ginkgolide C coculture with an AMPK activator (AICAR) affected AMPK phosphorylation and Sirt1 and ATGL protein levels, and (c) ginkgolide C coculture with an AMPK inhibitor (compound C) led to recovered AMPK phosphorylation and Sirt1 and ATGL protein production.

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