A Special Extract of Bacopa monnieri (CDRI-08)-Restored Memory in CoCl₂-Hypoxia Mimetic Mice Is Associated with Upregulation of Fmr-1 Gene Expression in Hippocampus

Table 1

Details of gene specific primer sequences, temperature conditions, cycle numbers and amplicon sizes.

Genes	Primers	PCR condition		Amplicon size
Hif-1a	F 5′-AGACAGACAAAGCTCATCCAAGG-3′; R 5′GCGAAGCTATTGTCTTTGGGTTTAA-3′	94°C - 3′		100 bp
		94°C - 45′′	30 cycles
		59°C - 30′′
		72°C - 45′′
Fmr-1	F 5′-TTACAGAAATAGGGGGCACG-3′; R 5′-TACGCTGTCTGGCTTTTCCT-3′	94°C - 3′		388 bp
		94°C - 45′′	34 cycles
		59°C - 30′′
		72°C - 45′′
Actb	F 5′-ATCGTGGGCCGCTCTAGGCACC-3′; R 5′CTCTTTGATGTCACGCACGATTTC-3′	94°C - 3′		543 bp
		94°C - 45′′	28 cycles
		57°C - 30′′
		72°C - 45′′