Potent Antiproliferative Effect on Liver Cancer of Medicinal Plants Selected from the Thai/Lanna Medicinal Plant Recipe Database “MANOSROI III”
Table 2
Antiproliferative activity on HepG2 cell line, DPPH radical scavenging and metal chelating activities, total phenolic and flavonoid contents of 9 methanolic extracts (MEs) and 36 semipurified fractions of the 9 selected medicinal plants selected from Thai/Lanna medicinal plant recipe database “MANOSROI III.”
Plant number
Name of medicinal plant
Hep G2
DPPH
Chelating
Phenolic content
Flavonoid content
IC50 (µg/mL)
Folds of
NCI criteria
Rank
SC50 (µg/mL)
Folds of
Rank
CC50 (µg/mL)
Folds of
Rank
QE (µg/mg)
Rank
QE (µg/mg)
Rank
C
D
T
A
E
3
Albizia chinensis ME
34.50
0.77
0.02
MA
23
59.30
0.40
0.31
8
>500
ND
16
218.51
6
0.81
28
A. chinensis HF
>100
ND
ND
IA
26
>100
ND
ND
12
435.60
0.03
14
12.98
39
ND
31
A. chinensis MF
>100
ND
ND
IA
26
40.20
0.59
0.46
4
>500
ND
16
149.17
9
3.95
19
A. chinensis BF
10.80
2.44
0.05
A
7
47.30
0.50
0.39
6
>500
ND
16
323.63
3
2.13
23
A. chinensis WF
31.50
0.84
0.02
MA
22
87.60
0.27
0.21
11
>500
ND
16
122.73
10
1.13
27
4
Caesalpinia sappan ME
20.12
1.31
0.03
MA
14
26.00
0.92
0.70
2
>500
ND
16
475.41
1
22.39
5
C. sappan HF
>100
ND
ND
IA
26
>100
ND
ND
12
>500
ND
16
22.90
31
ND
31
C. sappan MF
27.11
0.97
0.02
MA
20
21.91
1.09
0.84
1
>500
ND
16
61.58
18
9.61
10
C. sappan BF
9.97
2.65
0.05
A
6
26.60
0.89
0.69
3
>500
ND
16
361.69
2
4.62
15
C. sappan WF
>100
ND
ND
IA
26
>100
ND
ND
12
>500
ND
16
64.16
17
0.59
29
6
Fibraurea tinctoria ME
89.11
0.30
0.01
MA
25
43.20
0.55
0.42
5
>500
ND
16
305.17
4
2.32
22
F. tinctoria HF
>100
ND
ND
IA
26
>100
ND
ND
12
367.30
0.04
11
16.14
36
ND
31
F. tinctoria MF
19.11
1.38
0.03
A
12
51.10
0.47
0.36
7
>500
ND
16
155.36
8
4.15
17
F. tinctoria BF
21.33
1.24
0.03
MA
17
76.40
0.31
0.24
10
>500
ND
16
260.24
5
4.57
16
F. tinctoria WF
>100
ND
ND
IA
26
62.40
0.38
0.29
9
>500
ND
16
175.41
7
3.91
20
7
Gloriosa superba ME
20.11
1.31
0.03
MA
14
>100
ND
ND
12
>500
ND
16
52.83
20
8.32
11
G. superba HF
3.82
6.91
0.14
A
2
>100
ND
ND
12
>500
ND
16
20.00
32
ND
31
G. superba MF
0.53
50.07
1.02
A
1
>100
ND
ND
12
>500
ND
16
59.69
19
3.98
18
G. superba BF
14.73
1.79
0.04
A
9
>100
ND
ND
12
>500
ND
16
36.46
24
ND
31
G. superba WF
>100
ND
ND
IA
26
>100
ND
ND
12
336.60
0.04
8
8.61
41
ND
31
12
Pouzolzia pentandra ME
35.51
0.74
0.02
MA
24
>100
ND
ND
12
228.20
0.06
5
5.85
42
5.23
13
P. pentandra HF
18.36
1.44
0.03
A
11
>100
ND
ND
12
78.80
0.16
2
14.41
37
ND
31
P. pentandra MF
11.82
2.23
0.05
A
8
>100
ND
ND
12
>500
ND
16
46.38
22
16.24
7
P. pentandra BF
>100
ND
ND
IA
26
>100
ND
ND
12
>500
ND
16
26.15
28
0.13
30
P. pentandra WF
>100
ND
ND
IA
26
>100
ND
ND
12
>500
ND
16
5.30
44
ND
31
14
Rhinacanthus nasutus ME
30.91
0.85
0.02
MA
21
>100
ND
ND
12
264.10
0.05
7
13.72
38
12.76
8
R. nasutus HF
>100
ND
ND
IA
26
>100
ND
ND
12
130.60
0.10
3
17.39
33
ND
31
R. nasutus MF
4.90
5.38
0.11
A
3
>100
ND
ND
12
243.50
0.05
6
35.16
25
35.91
1
R. nasutus BF
>100
ND
ND
IA
26
>100
ND
ND
12
>500
ND
16
17.08
34
ND
31
R. nasutus WF
>100
ND
ND
IA
26
>100
ND
ND
12
398.40
0.03
13
9.24
40
1.85
24
15
Senna alata ME
22.46
1.18
0.02
MA
18
>100
ND
ND
12
>500
ND
16
67.68
16
21.84
6
S. alata HF
26.58
0.99
0.02
MA
19
>100
ND
ND
12
45.60
0.28
1
3.63
45
26.19
3
S. alata MF
5.75
4.59
0.09
A
5
>100
ND
ND
12
>500
ND
16
112.97
12
33.73
2
S. alata BF
>100
ND
ND
IA
26
>100
ND
ND
12
362.70
0.04
10
86.89
13
1.40
26
S. alata WF
>100
ND
ND
IA
26
>100
ND
ND
12
186.60
0.07
4
35.09
26
ND
31
19
Stemona colinsae
ME
5.51
4.79
0.10
A
4
>100
ND
ND
12
391.70
0.03
12
27.45
27
1.69
25
S. colinsae HF
>100
ND
ND
IA
26
>100
ND
ND
12
>500
ND
16
24.08
30
ND
31
S. colinsae MF
14.73
1.79
0.04
A
9
>100
ND
ND
12
>500
ND
16
44.83
23
4.82
14
S. colinsae BF
>100
ND
ND
IA
26
>100
ND
ND
12
>500
ND
16
16.82
35
ND
31
S. colinsae WF
>100
ND
ND
IA
26
>100
ND
ND
12
436.00
0.03
15
5.83
43
ND
31
23
Ventilago denticulata ME
20.88
1.26
0.03
MA
16
>100
ND
ND
12
>500
ND
16
83.73
14
10.08
9
V. denticulata HF
19.30
1.37
0.03
A
13
>100
ND
ND
12
>500
ND
16
48.75
21
26.00
4
V. denticulata MF
>100
ND
ND
IA
26
>100
ND
ND
12
>500
ND
16
120.09
11
7.25
12
V. denticulata BF
>100
ND
ND
IA
26
>100
ND
ND
12
>500
ND
16
78.36
15
2.63
21
V. denticulata WF
>100
ND
ND
IA
26
>100
ND
ND
12
343.20
0.04
9
25.14
29
ND
31
Standard
Cisplatin (C)
26.40
1.00
—
—
—
—
—
—
—
—
—
—
Doxorubicin (D)
0.54
—
1.00
—
—
—
—
—
—
—
—
—
α-Tocopherol (T)
—
—
—
—
—
23.80
1.00
0.77
—
NT
ND
—
Ascorbic acid (A)
—
—
—
—
—
18.30
1.30
1.00
—
NT
ND
—
EDTA (E)
—
—
—
—
—
NT
ND
ND
—
12.90
1.00
—
Folds of the standard drug were calculated as the ratio between the IC50 value of the standard drug and samples. NCI criteria: NCI criteria indicated three types of the activity. A: active (IC50 value was less than 20 µg/mL), MA: moderately active (IC50 value was between 20 and 100 µg/mL), and IA: inactive (IC50 value was more than 100 µg/mL). NT: not tested, ND: not determined. ME: methanolic extract, HF: -hexane fraction, MF: methanol-water fraction, BF: -butanol fraction, and WF: water fraction.
