Effect of Tongxinluo on Nephrin Expression via Inhibition of Notch1/Snail Pathway in Diabetic Rats

Figure 2

Effect of TXL on nephrin expression in diabetic rats and high glucose cultured podocyte. (a) Representative band of nephrin protein by western blot in rats. (b) Comparison of the grey value of nephrin protein in rats (). The result showed that nephrin protein was statistically significantly decreased in DN group compared with NC group (). Nephrin protein expression was significantly increased in TXL group compared with DN group (). (c) Representative immunohistochemical photograph for nephrin in vivo. Immunohistochemical results showed that nephrin protein expression was significantly decreased in DN group. (d) Comparison of mean optical density (MOD) of nephrin protein in rats. The result showed that nephrin protein was statistically significantly decreased in DN group compared with NC group (). Nephrin protein expression was significantly increased in TXL group compared with DN group (). (e) Representative band of nephrin protein by western blot in cultured podocyte. (f) Comparison of the grey value of nephrin protein in cultured podocyte (). The result showed that nephrin protein was statistically significantly decreased in HG group compared with NC group (). Nephrin protein expression was significantly increased in TXL group compared with HG group (). (g) Comparison of mRNA level of nephrin by RT-PCR in cultured podocyte (). The result showed that nephrin mRNA was statistically significantly decreased in HG group compared with NC group (). Nephrin mRNA expression was significantly increased in TXL group compared with HG group (). versus NG. versus HG.

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