Preliminary Studies of the Immunomodulator Effect of the Bougainvillea xbuttiana Extract in a Mouse Model

Figure 4

Vacuole formation. Peritoneal macrophages were obtained and exposed to 2.9, 29, and 290 μg/mL of Bxb as described above. After different times of incubation at 37°C in an atmosphere of 5% CO₂, the cells were stained with neutral red for 5 min. The absorbance was determined at 540 nm, and the results were expressed as described above. Each point represents the mean value of samples from four experiments in different groups of four mice. Statistical differences between the treatments were .