Cytotoxic Effects of Biosynthesized Zinc Oxide Nanoparticles on Murine Cell Lines

Table 3

Caspases spectrophotometric analysis of WEHI-3B cells after being treated with ZnO-NPs for 24, 48, and 72 h, respectively.

Cells %
Caspase	Control; 24 h	Treated; 24 h	Control; 48 h	Treated; 48 h	Control; 72 h	Treated; 72 h
Caspase-3	0.059 ± 0.030	0.11 ± 0.01^*	0.071 ± 0.051	0.16 ± 0.007^*	0.09 ± 0.0032	0.2 ± 0.006^*
Caspase-9	0.06 ± 0.071	0.15 ± 0.003^*	0.075 ± 0.001	0.18 ± 0.005^*	0.099 ± 0.002	0.235 ± 0.0035^*

Values are expressed as mean ± SD of three different experiments. The data has been analyzed using post hoc comparison test one-way ANOVA; means compared with Tukey’s test. ^*Significant ( < 0.05) increase of apoptotic cells in ZnO-NPs-treated groups compared with untreated controls.