The Roots of Atractylodes macrocephala Koidzumi Enhanced Glucose and Lipid Metabolism in C2C12 Myotubes via Mitochondrial Regulation

Figure 2

Effect of ARA extract on mitochondrial biogenesis/thermogenesis-related factors and ATP content. C2C12 cells were differentiated with DMEM containing 2% horse serum for 5 days and then treated with ARA extract (0.2, 0.5, and 1.0 mg/mL) or metformin (2.5 mM) for 24 h. The expressions of NRF, TFAM, and UCP3 were analyzed by RT-PCR (a). The values shown are ratios of mRNA GAPDH blot densities and are the means ± SEMs of three independent experiments (b). The total ATP content was measured using ATP calorimetric assay kit (c). Values in histogram are the means ± SEMs of three independent experiments. and versus nontreated differentiated cells.

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