The Roots of Atractylodes macrocephala Koidzumi Enhanced Glucose and Lipid Metabolism in C2C12 Myotubes via Mitochondrial Regulation

Figure 3

Effect of ARA extract on the phosphorylation of AMPK in C2C12 cells. C2C12 cells were differentiated with DMEM containing 2% horse serum for 5 days and then treated with ARA extract (0.2, 0.5, and 1.0 mg/mL) or metformin (2.5 mM) for 45 min. pAMPK, AMPK, pACC, and ACC protein levels were assessed by Western blotting (a) versus β-actin (the internal control). Values are expressed as the means ± SEMs of three independent experiments (b). and versus nontreated differentiated cells.

(a)

(b)