The Roots of Atractylodes macrocephala Koidzumi Enhanced Glucose and Lipid Metabolism in C2C12 Myotubes via Mitochondrial Regulation

Figure 4

Effect of ARA extract on the expression of SIRT1 in C2C12 cells. C2C12 cells were differentiated with DMEM containing 2% horse serum for 5 days and then treated with ARA extract (0.2, 0.5, and 1.0 mg/mL) or metformin (2.5 mM) for 24 h. SIRT1 mRNA (a) and protein (b) levels were assessed by RT-PCR and Western blotting, respectively. Histogram values are the means ± SEMs of three independent experiments. and versus nontreated differentiated cells.

(a)

(b)