The Roots of Atractylodes macrocephala Koidzumi Enhanced Glucose and Lipid Metabolism in C2C12 Myotubes via Mitochondrial Regulation

Figure 6

Effects of ARA extract on free fatty acid levels and CPT-1b expression in C2C12 cells. C2C12 cells were differentiated with DMEM containing 2% horse serum for 5 days and then treated with ARA extract (0.2, 0.5, and 1.0 mg/mL) or metformin (2.5 mM) for 24 h. FFA contents were measured in supernatants using a FFA assay (a). CPT-1b levels were assessed by RT-PCR (b). Histogram values are the means ± SEMs of three independent experiments. and versus nontreated differentiated cells.

(a)

(b)