Ferulic Acid Induces Th1 Responses by Modulating the Function of Dendritic Cells and Ameliorates Th2-Mediated Allergic Airway Inflammation in Mice

Figure 5

Anti-OVA antibody expression levels of ferulic acid-treated OVA-induced allergic asthmatic mice. (a) Brief description of the protocol of animal sensitization and challenge. On days 1, 14, and 28, all groups of mice were sensitized by an intraperitoneal injection of the OVA allergen. Three groups of mice (FA (25), FA (50), and FA (100)) were, respectively, orally fed 25, 50, and 100 mg/kg of FA on days 21 to 47. Positive control (PC) mice were administered sterilized water instead of FA. Then, mice were intranasally challenged with OVA on days 43 and 44. Subsequently, mice were exposed to OVA aerosols for 3 consecutive days, and AHR was measured 1 day after the last challenge. BALF was collected after measuring the AHR. Negative control (NC) mice were neither sensitized with OVA nor administered FA treatment but were challenged with OVA. (b) IgE, IgG₁, and anti-OVA antibody expressions of all groups of mice were measured by an ELISA. Results are expressed as the mean ± SEM ( in each group). , , and versus the PC group.

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