Orostachys japonicus Inhibits Expression of the TLR4, NOD2, iNOS, and COX-2 Genes in LPS-Stimulated Human PMA-Differentiated THP-1 Cells by Inhibiting NF-κB and MAPK Activation

Figure 6

Effects of O. japonicus on the LPS-induced phosphorylation of JNK, p38 MAPK, and ERK in THP-1 cells. Cells were pretreated for 20 h with various concentrations of O. japonicus (10 or 25 μg/mL) before exposure to LPS (1 μg/mL) for 4 h, and JNK, p38 MAPK, and ERK protein levels were determined by immunoblotting. Densitometric analyses are presented as the relative ratios of JNK (a) or p-JNK (d), p38 MAPK (b) or p-p38 MAPK (e), and ERK (c) or p-ERK (f) to β-actin. The data represent the means ± SD of three independent samples. and compared with LPS stimulation alone.

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