Evaluation of Antiradical and Anti-Inflammatory Activities of Ethyl Acetate and Butanolic Subfractions of Agelanthus dodoneifolius (DC.) Polhill & Wiens (Loranthaceae) Using Equine Myeloperoxidase and Both PMA-Activated Neutrophils and HL-60 Cells

Figure 2

Lucigenin-enhanced chemiluminescence assay. Effect of Agelanthus dodoneifolius subfractions on the chemiluminescence response of ROS generated by PMA-treated neutrophils cells. Data are expressed as mean ± SD of three parallel experiments. indicates significance compared to control solutions (one-way ANOVA followed by Dunnett’s posttest). NS: not stimulated cells. The IC₅₀ values were and  μg/mL, respectively, for the ethyl acetate and butanolic subfraction. The IC₅₀ value of gallic acid was  μg/mL. The letter (a) means that the IC₅₀ values were statistically similar (unpaired -test with Welch’s correction).