Resveratrol Protects PC12 Cell against 6-OHDA Damage via CXCR4 Signaling Pathway

Figure 1

6-OHDA induced damage to the cultured PC12 cells. (a–e) The photomicrographs of PC12 cells exposed to 6-OHDA of different concentration for 24 h. (a) PC12 cells in routine culture medium grew well with long neurites. (b) When exposed to 25 μM 6-OHDA, cell morphology had little changes. (c) When treated with 50 μM 6-OHDA, the neurites started to retract, and the neural network was collapsed in a certain degree with cell debris that appeared. (d and e) As the concentration of 6-OHDA increased to 100 and 150 μM, the cells were strongly insulted and severe cell loss could be observed. There was a mass of cell debris that could be found in the medium. Arrows indicated cell debris. (f) MTT assay also demonstrated that cell viability decreased gradually with the concentration of 6-OHDA increased. OD values were presented as means ± SD from five independent experiments. versus control group. Scale bars: (a–e) 100 μm.

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