Resveratrol Protects PC12 Cell against 6-OHDA Damage via CXCR4 Signaling Pathway

Figure 2

Protection of resveratrol on PC12 cells against 6-OHDA. (a) PC12 cells of normal control group grew in good condition and exhibited long neurites. (b) In 6-OHDA injury group, neurites were short and few with the neural network collapsed. (c–e) The cells were preincubated for 2 h with resveratrol at 12.5 (c), 25 (d), and 50 μM (e) before 50 μM 6-OHDA exposure, respectively. In 12.5 μM resveratrol group, the protective effect was weak with swollen cells and cell debris observed (c). 25 μM resveratrol promoted neurite growth, and only little cell debris could be found (d). However, in 50 μM resveratrol group, the alleviative effect was not obvious, and some cells swelled and turned into a round shape (e). Arrows (b and c) indicated cell debris. Cell viability was further measured by MTT assays (f). OD values were presented as means ± SD from five independent experiments. versus 6-OHDA injury group. Scale bars: (a–d) 100 μm.

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