Triterpenoids and Polysaccharide Fractions of Ganoderma tsugae Exert Different Effects on Antiallergic Activities

Figure 3

The relative Th2 cytokines (a) and Th1 cytokines (b) productions from primary T cells cultured with different doses of triterpenoids and polysaccharide extracts from G. tsugae. Isolated CD4⁺ helper T cells ( cells/mL) from the DO11.10 transgenic mice with high percentage of OVA peptide-specific T cell receptor were cultured with different doses of Gt-TRE or Gt-PS in the presence of OVA peptide and APC for 72 hrs. The supernatants were collected and cytokine levels were determined by ELISA method. The results are expressed as the mean ± SD of four independent experiments. as compared to the 0 μg/mL control cells.

(a) Th2 cytokines production

(b) Th1 cytokine production