Triterpenoids and Polysaccharide Fractions of Ganoderma tsugae Exert Different Effects on Antiallergic Activities

Figure 5

The NF-κB transcriptional activity and IL-4 production by activated transfected EL4 T cells cultured with different doses of triterpenoids and polysaccharide extracts from G. tsugae. EL4 T cells ( cells/mL) transiently transfected with NF-κB-promoted luciferase reporter plasmid were treated with Gt-TRE or Gt-PS and stimulated with 50 ng/mL PMA plus 1 μg/mL ionomycin for 24 hrs. Mocks are the EL4 cells transfected with empty vector as the negative control without Gt-TRE or Gt-PS treatment. The data are representative for five independent experiments. , as compared to the 0 μg/mL control cells.

(a) Luciferase activity

(b) IL-4 production