Research Article

Inhibitory Effects of Cytosolic Ca2+ Concentration by Ginsenoside Ro Are Dependent on Phosphorylation of IP3RI and Dephosphorylation of ERK in Human Platelets

Table 1

Effects of Rp-8-Br-cAMPS and Rp-8-Br-cGMPS on [Ca2+ mobilization.

[Ca2+ (nM)Increase (%)

G-Ro (155 μM) + thrombin (0.05 U/mL)160.3 ± 8.1
G-Ro (155 μM) + Rp-8-Br-cAMPS (250 μM) + thrombin (0.05 U/mL)326.3 ± 9.0103.6(1)
G-Ro (155 μM) + Rp-8-Br-cGMPS (250 μM) + thrombin (0.05 U/mL)202.6 ± 4.626.4(2)

G-Ro (300 μM) + thrombin (0.05 U/mL)140.1 ± 1.8
G-Ro (300 μM) + Rp-8-Br-cAMPS (250 μM) + thrombin (0.05 U/mL)260.3 ± 9.985.8(1)
G-Ro (300 μM) + Rp-8-Br-cGMPS (250 μM) + thrombin (0.05 U/mL)191.8 ± 7.336.9(2)

Data were from Figures 4(b) and 4(c). (1) Increase (%) = [(G-Ro + thrombin + Rp-8-Br-cAMPS) − (G-Ro + Thrombin)]/(G-Ro + thrombin) × 100, (2) increase (%) = [(G-Ro + thrombin + Rp-8-Br-cGMPS) − (G-Ro + thrombin)]/(G-Ro + thrombin) × 100.