Adenosine A2A Receptors Mediate Anti-Inflammatory Effects of Electroacupuncture on Synovitis in Mice with Collagen-Induced Arthritis

Figure 6

Immunohistochemical detection of R in total ankle joints. Immunohistochemical analysis of joint sections from CIA-control mice revealed positive staining for R in contrast to Sham-control mice (b, a), which was localized primarily in the inflammatory synovial tissue, and mainly on synovial fibroblasts (f, g), chondrocytes (h, i), and inflammatory cells (j). The staining for R in CIA-EA mice was similar to that in CIA-control mice (b, c), while there was less inflammatory cell infiltration, synovial hyperplasia, and bone erosion (Figure 4). However, the staining for R was significantly increased in CIA-EA-SCH58261 mice (d) and in CIA-SCH58261 mice (e). Values (IOD in %) are means ± SEM of 10 animals for each group. versus Sham-control. ^# versus CIA-EA (k). Magnification ×200 (a, b, c, d, and e); ×400; (+) represented the positive staining, while (−) represented negative staining. (f, g, h, i, and j).

(a) Sham-control

(b) CIA-control

(c) CIA-EA

(d) CIA-EA-SCH58261

(e) CIA-SCH58261

(f) Synovial fibroblasts (−)

(g) Synovial fibroblasts (+)

(h) Chondrocytes (−)

(i) Chondrocytes (+)

(j) Inflammatory cell (+)

(k)