Research Article

Primo Vascular System: An Endothelial-to-Mesenchymal Potential Transitional Tissue Involved in Gastric Cancer Metastasis

Figure 1

Isolation of stable, high GFP expressed MKN-45-GFP cells. (a) The enhanced GFP4 expression plasmid was transfected MKN-45 cells. The brightest fluorescent cells were sorted and cloned by flow cytometry. Stable high GFP-expression clones were isolated and cultured in the absence of G418 for 10 passages. (b) Stable high GFP-expression MKN-45-GFP cells cultured in the absence of G418 for 20 passages.
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