Methanolic Extract of Clinacanthus nutans Exerts Antinociceptive Activity via the Opioid/Nitric Oxide-Mediated, but cGMP-Independent, Pathways

Figure 3

Effect of MECN on acetic acid-induced abdominal constriction in mice. Animals were treated with vehicle (10 mL/kg, p.o.), ASA (100 mg/kg, p.o.), or MECN (100, 250, and 500 mg/kg, p.o.) 60 min before acetic acid (0.6%, 10 mL/kg, i.p.) treatment. Naloxone (Nalox, 5 mg/kg, i.p.) was administered 15 min before MECN (500 mg/kg, p.o.) or vehicle (10 mL/kg, p.o.). Each column represents the mean ± SEM of six mice. Statistical analyses were performed using 1-way ANOVA followed by Tukey’s post hoc test. , compared to control group; compared to 500 mg/kg MECN-treated group. Values in parentheses denote percentage of inhibition.