Effects of Guchang Capsule on Dextran Sulphate Sodium-Induced Experimental Ulcerative Colitis in Mice

Figure 4

GC treatment decreased proinflammatory cytokines production in LPS stimulated RAW264.7 cells. (a) ELISA analysis of secreted proinflammatory cytokines; (b) qPCR analysis of mRNA level of proinflammatory cytokines. RAW264.7 cells were stimulated with LPS (100 ng/mL) together with GC (1 mg/mL) for 4 hours and the protein level (a) or mRNA level (b) of TNFα, IL-6, and IL-1β was analyzed. The data are representative of three independent experiments (mean ± SD). versus control + DSS group; versus PBS + control group.

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