Effects of Guchang Capsule on Dextran Sulphate Sodium-Induced Experimental Ulcerative Colitis in Mice

Figure 5

GC suppressed NF-κB activation after LPS stimulation in RAW264.7 cells. (a) Luciferase reporter assay; (b) Western blot analysis; (c) quantification of protein level. RAW264.7 cells were transfected with NF-κB luciferase reporter plasmid together with pRL-TK-Renilla-luciferase plasmid (internal control); 24 hours later the cells were stimulated with LPS (100 ng/mL) together with GC (1 mg/mL) for 4 hours; the luciferase reporter assay was performed to examine the activation of NF-κB promoter (a); versus control + DSS group; versus PBS + control group. The expressions of p-p65, p65, p-IKKβ, IKKβ, p-IκBα, and IκBα were examined by WB in RAW264.7 cells after stimulation with LPS and treatment with different quality of GC (b). Quantification of protein level of p-p65, p-IKKβ, p-IκBα in (b) and (c); versus control + LPS group, versus control + LPS group, versus control + LPS group, and versus untreated control group. The data are representative of three independent experiments (mean ± SD in (a, c)).

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